Autoclave was the science officer appointed by Starscream to run the Dark Energon Facility aboard Trypticon Station.
When Megatron's Gladiator Corps invaded Trypticon, killing many of Starscream's scientists, Autoclave was spared to participate in an experiment. Two subjects were selected and forced to ingest Dark Energon, with explosive results. Shockwave complimented Autoclave on refining Dark Energon from its original state when he got the necessary ingots, to which Autoclave impolitely replied they had simply succeeded. He was knocked out by security charges as the effects of Dark Energon electrified the room, but recovered soon enough to explain to Megatron that more Dark Energon could be made by polluting it into regular Energon.